| Strain Name |
|
Stock No. | 110141 |
| Common name |
B-Tcf21-iCreERT2 mice |
Gene symbol and name | Tcf21 (transcription factor 21), Po, ep |
| Strain of origin |
C57BL/6 |
Chromosome | 10 |
| Coat color | Black | Dietary information | Growth and reproduction diet for experimental mice |
|
NCBI Gene ID |
21412 | ||
Application
- Function research of genes
- This Tcf21iCreERT2 model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of Transcription factor 21 expressed during mouse embryogenesis specifically in mesodermally-derived cells that surround the epithelium of the developing gastrointestinal, genitourinary, and respiratory systems, and may be useful for studying mouse embryonic development.
Expression
Expressed gene
Cre, cre recombinase, bacteriophage P1
Site of expression
Bladder adult, Lung adult, mouse embryos in mesenchymal cells
Gene editing strategy
A iCreERT2-WPRE-pA sequence cassette was inserted downstream 5’UTR of the Tcf21 gene. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
Figure
2. Blocking SEV secretion from ECs delays AML progression. (A) The
experimental design. (B–F) Survival curves of AML recipients
with different genotypes. Cdh5-Cre;Vps33bfl/fl (B), Lepr-Cre;Vps33bfl/fl (C),
Osx-CreER;Vps33bfl/fl (D),
Pf4-Cre;Vps33bfl/fl (E), and
Tcf21-CreER;Vps33bfl/fl (F) are
shown (n = 5–8; **P < 0.01, ***P < 0.001, log-rank
test). Experiments were conducted 3 times for validation.
They used 7 cell type–specific mouse Cre lines to conditionally knock out Vps33b in Cdh5+ or Tie2+ endothelial cells (ECs), Lepr+ BM perivascular cells, Osx+ osteoprogenitor cells, Pf4+ megakaryocytes, and Tcf21+ spleen stromal cells.
(source: Huang D et al.,2021)
reference
1. Huang D, Sun G, Hao X, He X, Zheng Z, Chen C, Yu Z, Xie L, Ma
S, Liu L, Zhou BO, Cheng H, Zheng J, Cheng T. ANGPTL2-containing small
extracellular vesicles from vascular endothelial cells accelerate leukemia
progression. J Clin Invest. 2021 Jan 4;131(1):e138986. [PubMed: 33108353]
