| Strain Name |
|
Stock No. | 110131 |
| Common name |
B-Sp7-F2A-iCre mice |
Gene symbol and name | Sp7(transcription factor 7), Osx |
| Strain of origin |
C57BL/6 |
Chromosome | 15 |
| Coat color | Black | Dietary information | Growth and reproduction diet for experimental mice |
|
NCBI Gene ID |
170574 | ||
Application
- Function research of genes
- This Sp7iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of bone development, osteoblast lineage, and Hedgehog/Wnt signaling.
Expression
Expressed gene
iCre, improved cre recombinase, bacteriophage P1
Site of expression
osteoblast lineage, embryos perichondrium, bone trabeculae, bone collars
Gene editing strategy
An F2A-iCre sequence cassette was placed between the coding sequence of exon 2 and 3’UTR of the Sp7 gene in C57BL/6 ES cells. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
Fig 4. qPCR, Western blot, and Immunofluorescence analyses showed that Fam20c knockout resulted in a decreased expression of Calpastatin in OB Fam20cKO cells (Fig. 4A, B, E), whereas accompanied by a decreased phosphorylated expression level of Calpastatin (Fig. 4C). These were consistent with the results of proteomics and phosphoproteomics. Furthermore, Fam20cdeficient osteoblasts displayed a diminished expression of Calpain 1 and Calpain 2 (Fig. 4A, B, E) and attenuation of Calpain activity (Fig. 4D).
(source: Liu et al. Journal of Translational Medicine (2023) 21:417)
Reference
1. Rodda SJ; McMahon AP. 2006. Distinct roles
for Hedgehog and canonical Wnt signaling in specification, differentiation and
maintenance of osteoblast progenitors. Development 133(16):3231-44. [PubMed:
16854976]
2. Canalis E; Parker K; Feng JQ; Zanotti S.
2013. Osteoblast lineage-specific effects of notch activation in the skeleton.
Endocrinology 154(2):623-34. [PubMed: 23275471]
[MGI Ref ID J:194661] Canalis E; Parker K; Feng JQ; Zanotti S. 2013.
Osteoblast lineage-specific effects of notch activation in the skeleton.
Endocrinology 154(2):623-34. [PubMed: 23275471]
3. Chen J; Shi Y; Regan J; Karuppaiah K; Ornitz
DM; Long F. 2014. Osx-Cre targets multiple cell types besides osteoblast
lineage in postnatal mice. PLoS One 9(1):e85161. [PubMed: 24454809]
4. Liu Y; Strecker S; Wang L; Kronenberg MS;
Wang W; Rowe DW; Maye P. 2013. Osterix-cre labeled progenitor cells contribute
to the formation and maintenance of the bone marrow stroma. PLoS One
8(8):e71318. [PubMed: 23951132]
5. Shekaran A; Shoemaker JT; Kavanaugh TE; Lin
AS; LaPlaca MC; Fan Y; Guldberg RE; Garcia AJ. 2014. The effect of conditional
inactivation of beta 1 integrins using twist 2 Cre, Osterix Cre and osteocalcin
Cre lines on skeletal phenotype. Bone 68:131-41. [PubMed: 25183373]
