Strain specific analysis of GPC3 gene expression in wild type (WT) mice and B-hGPC3 mice by RT-PCR. Mouse Gpc3 mRNA was detectable only in kidney of WT mice (+/+). Human GPC3 mRNA was detectable only in homozygous B-hGPC3 mice (H/H) but not in WT mice (+/+). 

Strain specific GPC3 expression analysis in homozygous B-hGPC3 mice by western blot. Kidney, heart and brain were collected from wild type (WT) mice (+/+) and homozygous B-hGPC3 mice (H/H), and analyzed by western blot with anti-GPC3 antibody. GPC3 was detectable in WT mice (+/+) and homozygous B-hGPC3 mice (H/H) due to the cross-reactivity of antibodies.