Strain specific analysis of GIPR mRNA expression in wild-type C57BL/6 mice and B-hGIPR mice by RT-PCR. Stomach RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hGIPR mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GIPR primers. Mouse Gipr mRNA was detectable only in wild-type C57BL/6 mice. Human GIPR mRNA was detectable only in homozygous B-hGIPR mice but not in wild-type mice. 

Western blot analysis of GIPR protein expression in homozygous B-hGIPR mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hGIPR mice (H/H), and then analyzed by western blot with anti-GIPR antibody. 40 μg total proteins were loaded for western blotting analysis. GIPR was detected in brain, stomach, pancreas and liver.

Representative GIPR expression in different tissues of B-hGIPR mice by IHC. Tissues were stained with antibodies that recognized with both human and mouse GIPR (B-J) or anti-IgG antibody (A). Pancreas and brain of B-hGIPR mice and wild-type C57BL/6 mice show GIPR positive. Liver and adipose of B-hGIPR mice and wild-type C57BL/6 mice show GIPR negative. Mouse tissues from the wild-type C57BL/6 mice (A-E). Human pancreas carcinoma as a positive control (F). Other tissues were got from homozygous B-hGIPR mice (G-J). Original magnification ×200. Abbreviations: IHC, immunohistochemistry.